Primary Identifier | MGI:7738743 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Tcf23 |
Strain of Origin | C57BL/6N | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 genome editing technology was used to create a deletion of the three coding exons between the 5'UTR and 3'UTR. RT-PCR analysis showed no mRNA expression in uterine tissues from homozygous mutant females. Western bot analysis confirmed the absence of protein expression in the uterus. |