| Primary Identifier | MGI:7767936 | Allele Type | Endonuclease-mediated |
| Attribute String | Transactivator | Gene | Ednrb |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The Ednrb-T2A-rTA strain was generated by the Genetic Modification Service at the Francis Crick Institute using CRISPR-Cas9 assisted targeting to insert a dox-inducible reverse transactivator into exon 8 of the Ednrb gene via a GSG and T2A peptide linker and followed by a stop codon. The donor template contained a 774bp insert of GSG, T2A and rtTA3, with 692bp of homology at the 5' end and 700bp homology at the 3' end. The AAV donor vector was synthesised and packaged into AAV serotype 1 by VectorBuilder. The guide sequence used was 5'-ATAAATACAGCTCGTCTTGA-3', that was synthesised as a synthetic guide RNA by IDTTM. |
