| Primary Identifier | MGI:7798887 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Ezh2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A mini-gene comprising wild type exons 16â20 and flanked by loxP sites was inserted into the endogenous intron 15â16 of the gene followed by an frt-flanked neomycin selection cassette. A point mutation (TAC/TTC; aa, T > P) was engineered in exon 16 of the endogenous gene. The neomycin cassette was removed via FLP-mediated recombination in the germline. This allele expresses wild-type protein prior to cre-mediated recombination. Upon removal of the mini-gene, a mutant protein is expressed which contains the exon 16 mutation. |
