| Primary Identifier | MGI:7856631 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Mettl3 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying Mettl3tm1Jcbr allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). The L1L2_Bact_P cassette was inserted at position 52535930 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 52536652. After flp recombination, the critical exon 4 is thus flanked by loxP sites. |
