| Primary Identifier | MGI:7856673 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Dp(17Airn)1Arge |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was made by chromosome engineering by targeted meiotic recombination (Herault et al 1998), by breeding together the Airn truncation allele (Sleutels et al 2002 Nature) and the Igf2r promoter deletion (Sleutels et al 2003 EMBO J.) with Hprt-Cre (Tang et al 2002 Genesis), and screening the offspring of triple mutant males for recombination events. |
| molecularNote | The allele has a 23kb duplication on chromosome 17 between 3kb downstream of the Airn transcription start site (TSS) (Igf2r intron 2) and 1.9kb downstream of the Igf2r TSS (GRCm38/mm10, chr17: 12,744,358 -12,767,833). This results in an extra 23kb of Airn transcription before it reaches the Igf2r promoter, and duplication of exon 2 of Igf2r that is predicted to cause a frame-shift. |
