| Primary Identifier | MGI:7859338 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cst7 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A construct containing a loxP site, FRT-flanked pgk-neo cassette, exon 2 containing two amino acid substitutions (C25A, C62A), and second loxP site was inserted in place of the endogenous exon 2 via homologous recombination. Recombinase-mediated recombination removed the neo cassette and exon 2. Immunoblot analysis confirmed the absence of protein expression in homozygous mice. |
