| First Author | Barbehenn EK | Year | 1974 |
| Journal | Proc Natl Acad Sci U S A | Volume | 71 |
| Issue | 4 | Pages | 1056-60 |
| PubMed ID | 4275392 | Mgi Jnum | J:318723 |
| Mgi Id | MGI:6861806 | Doi | 10.1073/pnas.71.4.1056 |
| Citation | Barbehenn EK, et al. (1974) The explanation for the blockade of glycolysis in early mouse embryos. Proc Natl Acad Sci U S A 71(4):1056-60 |
| abstractText | The reason for the failure of early-stage mouse embryos to grow on glucose alone was investigated by measurement of glucose-6-phosphate, fructose-1,6-bisphosphate plus triose phosphates, citrate, and malate in individual embryos during starvation and refeeding with glucose or glucose plus pyruvate. The results indicate a block at the 6-phosphofructokinase (EC 2.7.1.11) step at early stages, which is later removed. Although there seems to be no early difficulty in phosphorylation of glucose, maximum glucose-6-phosphate levels (and probably fructose-6-phosphate levels) are much lower at early stages than at later stages. The increase in fructose-6-phosphate with age may be the major cause of the increase in 6-phosphofructokinase activity. Unusually high citrate levels at all ages may help to keep this enzyme strongly inhibited until the increase in fructose-6-phosphate occurs. The changes in metabolite levels also indicate an early defect in mobilization of glycogen and a probably less important defect in the citrate cycle. |
