| First Author | Wakasugi K | Year | 2010 |
| Journal | FEBS Lett | Volume | 584 |
| Issue | 1 | Pages | 229-32 |
| PubMed ID | 19941862 | Mgi Jnum | J:155529 |
| Mgi Id | MGI:4414690 | Doi | 10.1016/j.febslet.2009.11.073 |
| Citation | Wakasugi K (2010) Species-specific differences in the regulation of the aminoacylation activity of mammalian tryptophanyl-tRNA synthetases. FEBS Lett 584(1):229-32 |
| abstractText | Tryptophanyl-tRNA synthetases (TrpRSs) catalyze the aminoacylation of tRNA(Trp). Previously, I demonstrated that Zn(2+)-depleted human TrpRS is enzymatically inactive and that binding of Zn(2+) or heme to human TrpRS stimulates its aminoacylation activity. In the present study, bovine and mouse TrpRSs were found to be constitutively active regardless of the presence of Zn(2+) or ferriprotoporphyrin IX chloride. Mutagenesis experiments demonstrated that the human H130R mutant is constitutively active and that the bovine R135H, E438A double mutant binds with Zn(2+) or heme to enhance its aminoacylation activity as does human wild-type TrpRS. These results provide the first evidence of species-specific regulation of TrpRS activity. |
