| First Author | Ji QS | Year | 1998 |
| Journal | Mol Biol Cell | Volume | 9 |
| Issue | 4 | Pages | 749-57 |
| PubMed ID | 9529375 | Mgi Jnum | J:48234 |
| Mgi Id | MGI:1267000 | Doi | 10.1091/mbc.9.4.749 |
| Citation | Ji QS, et al. (1998) Epidermal growth factor signaling and mitogenesis in Plcg1 null mouse embryonic fibroblasts. Mol Biol Cell 9(4):749-57 |
| abstractText | Gene targeting techniques and early mouse embryos have been used to produce immortalized fibroblasts genetically deficient in phospholipase C (PLC)-gamma1, a ubiquitous tyrosine kinase substrate. Plcg1(-/-) embryos die at embryonic day 9; however, cells derived from these embryos proliferate as well as cells from Plcg1(+/+) embryos. The null cells do grow to a higher saturation density in serum-containing media, as their capacity to spread out is decreased compared with that of wild-type cells. In terms of epidermal growth factor receptor activation and internalization, or growth factor induction of mitogen-activated protein kinase, c-fos, or DNA synthesis in quiescent cells, PLcg1(-/-) cells respond equivalently to PLcg1(+/+) cells. Also, null cells are able to migrate effectively in a wounded monolayer. Therefore, immortalized fibroblasts do not require PLC-gamma1 for many responses to growth factors. |
