| First Author | COFFEY JW | Year | 1965 |
| Journal | Arch Biochem Biophys | Volume | 110 |
| Pages | 117-23 | PubMed ID | 14321840 |
| Mgi Jnum | J:319880 | Mgi Id | MGI:6866947 |
| Doi | 10.1016/0003-9861(65)90162-1 | Citation | Coffey JW, et al. (1965) Studies on the Interaction of Vitamin B12: Intrinsic Factor and Receptors. Arch Biochem Biophys 110:117-123 |
| abstractText | The chemical and biological properties of vitamin B12 combining substances in mouse stomach extracts (MIF), mouse serum (MS), and mouse ascites fluid (AS) were compared. MIF was found to enhance the absorption of vitamin B12 by rings of mouse and guinea pig ileum and to inhibit its absorption by sarcoma-180 (S-180) cells in vitro. :\IS and AS, on the other hand, enhanced the absorption of vitamin B12 by S-180 cells but inhibited its absorption by rings of mouse and guinea pig ileum. It was shown that MIF is capable of forming a stable complex with vitamin B12 at pH values from 1.0 to 11.0, while MS and AS are not capable of forming such stable complexes at pH values below 5.0. The complex formed between vitamin B12 and MIF (MIF-vitamin B!2) is not adsorbed by zirconyl phosphate gel (Z-gel) at pH values higher than 5.0; however, the complexes formed between vitamin B12 and MS (MSÂvitamin B12) or AS (AS-vitamin B12) are adsorbed by Z-gel at pH values as high as 7.0. By chromatography on CM-cellulose, it was possible to separate MIF-vitamin B12 from MS-vitamin B12 and AS-vitamin B12- The latter two complexes behaved idenÂtically when chromatographed on C:\f-cellulose. |
