| First Author | Hao Z | Year | 1999 |
| Journal | Dev Dyn | Volume | 216 |
| Issue | 2 | Pages | 201-17 |
| PubMed ID | 10536059 | Mgi Jnum | J:57955 |
| Mgi Id | MGI:1346241 | Doi | 10.1002/(SICI)1097-0177(199910)216:2<201::AID-DVDY10>3.0.CO;2-6 |
| Citation | Hao Z, et al. (1999) Differential expression of Hoxa-2 protein along the dorsal-ventral axis of the developing and adult mouse spinal cord. Dev Dyn 216(2):201-17 |
| abstractText | We have used synthetic oligopeptides derived from the coding sequence of the murine Hoxa-2 protein to produce polyclonal antibodies that specifically recognize the Hoxa-2 recombinant protein. Immunohistochemical studies reveal a distinct pattern of spatial and temporal expression of Hoxa-2 protein within the mouse spinal cord which is concomitant with the cytoarchitectural changes occurring in the developing cord. Hoxa-2 protein is predominantly detected in the nuclei of cells in the ventral mantle region of 10-day-old mouse embryos. Islet-1, a marker for motor neurons was also shown to be co-localized with Hoxa-2 in nuclei of cells in this region. As development progresses from 10-days to 14-days of gestation, Hoxa-2 protein expression gradually extends to the dorsal regions of the mantle layer. The Hoxa-2 protein expression pattern changes at 16-days of embryonic development with strong expression visible throughout the dorsal mantle layer. In 18-day-old and adult mouse spinal cords, Hoxa-2 protein was expressed predominantly by cells of the dorsal horn and only by a few cells of the ventral horn. Double labeling studies with an antibody against glial fibrillary acidic protein (GFAP, an astrocyte-specific intermediate filament protein) showed that within the adult spinal cord, astrocytes rarely expressed the Hoxa-2 protein. However, Hoxa-2 and GFAP double-labeled astrocytes were found in the neopallial cultures, although not all astrocytes expressed Hoxa-2. Hoxa-2 expressing oligodendrocyte progenitor cells were also identified after double-labeling with O4 and Hoxa-2 antibodies; although cells in this lineage that have begun to develop a more extensive array of cytoplasmic processes were less likely to be Hoxa-2 positive. The early pattern of Hoxa-2 protein expression across transverse sections of the neural tube is temporally and spatially modified as each major class of neuron is generated. This congruence in the expression of the Hoxa-2 protein and the generation of neurons in the cord suggests that the Hoxa-2 protein may contribute to dorsal-ventral patterning and/or to the specification of neuronal phenotype. Dev Dyn 1999;216:201-217. Copyright 1999 Wiley-Liss, Inc. |
