| First Author | Oda S | Year | 2000 |
| Journal | EMBO J | Volume | 19 |
| Issue | 7 | Pages | 1711-8 |
| PubMed ID | 10747038 | Mgi Jnum | J:61445 |
| Mgi Id | MGI:1354996 | Doi | 10.1093/emboj/19.7.1711 |
| Citation | Oda S, et al. (2000) Efficient repair of A/C mismatches in mouse cells deficient in long-patch mismatch repair. EMBO J 19(7):1711-8 |
| abstractText | A previously unrecognized mismatch repair activity is described. Extracts of immortalized MSH2-deficient mouse fibroblasts did not correct most single base mispairs. The same extracts carried out efficient repair of A/C mismatches. A/G mispairs were less efficiently corrected and there was no significant repair of A/A. MLH1-defective mouse extracts also repaired an A/C mispair. A/C correction by Msh2(-/-) mouse cell extracts was not affected by antibodies against the PMS2 protein, which inhibited long-patch mismatch repair. A/C repair activity is thus independent of MutSalpha, MutSbeta and MutLalpha. A/C mismatches were corrected 5-fold more efficiently by extracts of Msh2 knockout mouse cells than by comparable extracts prepared from hMSH2- or hMLH1-deficient human cells. MSH2-independent A/C correction by mouse cell extracts did not require a nick in the circular duplex DNA substrate. Repair involved replacement of the A and was associated with the resynthesis of a limited stretch of </=25 bases of DNA. |
