| First Author | Perez-Reyes E | Year | 1992 |
| Journal | J Biol Chem | Volume | 267 |
| Issue | 3 | Pages | 1792-7 |
| PubMed ID | 1370480 | Mgi Jnum | J:28892 |
| Mgi Id | MGI:76430 | Doi | 10.1016/s0021-9258(18)46015-2 |
| Citation | Perez-Reyes E, et al. (1992) Cloning and expression of a cardiac/brain beta subunit of the L-type calcium channel. J Biol Chem 267(3):1792-7 |
| abstractText | The skeletal muscle dihydropyridine receptor/Ca2+ channel is composed of five protein components (alpha 1, alpha 2 delta, beta, and gamma). Only two such components, alpha 1 and alpha 2, have been identified in heart. The present study reports the cloning and expression of a novel beta gene that is expressed in heart, lung, and brain. Coexpression of this beta with a cardiac alpha 1 in Xenopus oocytes causes the following changes in Ca2+ channel activity: it increases peak currents, accelerates activation kinetics, and shifts the current-voltage relationship toward more hyperpolarized potentials. It also increases dihydropyridine binding to alpha 1 in COS cells. These results indicate that the cardiac L-type Ca2+ channel has a similar subunit structure as in skeletal muscle, and provides evidence for the modulatory role of the beta subunit. |
