| First Author | Krizanova O | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 211 |
| Issue | 3 | Pages | 921-7 |
| PubMed ID | 7598723 | Mgi Jnum | J:26511 |
| Mgi Id | MGI:73956 | Doi | 10.1006/bbrc.1995.1900 |
| Citation | Krizanova O, et al. (1995) Coexpression of skeletal muscle voltage-dependent calcium channel alpha 1 and beta cDNAs in mouse Ltk- cells increases the amount of alpha 1 protein in the cell membrane. Biochem Biophys Res Commun 211(3):921-7 |
| abstractText | Coexpression of alpha 1 and beta subunits for all tissue-specific isotypes of calcium channels results in an increase in Ca2+ channel current density and drug receptor function. The quantification of the photoaffinity labeled skeletal muscle alpha 1 subunit polypeptide by two alternative immunoadsorption techniques showed that 3 times higher amount of calcium channel alpha 1 protein appears in the cell membrane when alpha 1 and beta subunits were coexpressed compared to alpha 1 alone. Using Western blotting and immunodetection techniques, we identified two polypeptide bands of alpha 1 with 210 kDa and 170 kD molecular mass, respectively. The combined intensity of these bands was 7-9 times higher when alpha 1 and beta were coexpressed compared to alpha 1 alone. The data provide evidence that coexpression of alpha 1 and beta results in an increased amount of alpha 1 protein in the cell membrane. |
