| First Author | Stanfield PR | Year | 1994 |
| Journal | J Physiol | Volume | 475 |
| Issue | 1 | Pages | 1-7 |
| PubMed ID | 8189383 | Mgi Jnum | J:18355 |
| Mgi Id | MGI:66351 | Doi | 10.1113/jphysiol.1994.sp020044 |
| Citation | Stanfield PR, et al. (1994) The intrinsic gating of inward rectifier K+ channels expressed from the murine IRK1 gene depends on voltage, K+ and Mg2+. J Physiol 475(1):1-7 |
| abstractText | 1. We describe the cloning of the inward rectifier K+ channel IRK1 from genomic DNA of mouse; the gene is intronless. 2. The IRK1 gene can be stably expressed in murine erythroleukaemia (MEL) cells. Such transfected cells show inward rectification under whole-cell recording. 3. Channels encoded by the IRK1 gene have an intrinsic gating that depends on voltage and [K+]o. Rate constants are reduced e-fold as the driving force on K+(V-EK) is reduced by 24.1 mV. 4. Removal of intracellular Mg2+ permits brief outward currents under depolarization. The instantaneous current-voltage relation may be fitted by an appropriate constant field expression. 5. Removal of intracellular Mg2+ speeds channel closure at positive voltages. In nominally zero [Mg2+]i, rate constants for the opening and closing of channels, processes which are first order, are similar to those of native channels. |
