| First Author | Joly EC | Year | 1994 |
| Journal | J Cell Sci | Volume | 107 ( Pt 10) |
| Pages | 2851-9 | PubMed ID | 7876352 |
| Mgi Jnum | J:21192 | Mgi Id | MGI:69224 |
| Doi | 10.1242/jcs.107.10.2851 | Citation | Joly EC, et al. (1994) TRiC-P5, a novel TCP1-related protein, is localized in the cytoplasm and in the nuclear matrix. J Cell Sci 107(Pt 10):2851-9 |
| abstractText | We have recently reported the cloning of a novel protein, TRiC-P5, with significant homology with protein 1 of the t-complex (TCP1). In the present study, the cellular localization of TRiC-P5 in Raji cells has been determined using an antiserum raised against a 18.5 kDa fusion protein. Results from cell fractionation and immunoblot studies indicate that TRiC-P5 is mainly localized in the cytoplasm. In addition, a significant part of TRiC-P5 is also found in the nucleus where it is attached to the nuclear matrix, a complex filament network involved in essential cellular functions such as DNA replication, and RNA transcription and maturation. Immunofluorescence experiments using the anti-TRiC-P5 antibodies confirm these results. We also provide evidence that, in the cytoplasm, TRiC-P5 is part of a large protein complex, most probably the TCP1-ring complex (TRiC), a hetero-oligomeric ring complex that plays a role of molecular chaperone in the folding of actin and tubulin. |
