| First Author | Parr T | Year | 2001 |
| Journal | Arch Biochem Biophys | Volume | 395 |
| Issue | 1 | Pages | 1-13 |
| PubMed ID | 11673859 | Mgi Jnum | J:72662 |
| Mgi Id | MGI:2153375 | Doi | 10.1006/abbi.2001.2546 |
| Citation | Parr T, et al. (2001) Calpastatin expression in porcine cardiac and skeletal muscle and partial gene structure. Arch Biochem Biophys 395(1):1-13 |
| abstractText | The expression in porcine skeletal and cardiac muscle of calpastatin, the specific endogenous inhibitor of the calpain proteolytic system, was examined 16 h after a single dose of a specific beta(2)-agonist. Immunoblotting of extracts indicated that treatment increased skeletal calpastatin 135-kDa band intensity (P < 0.01), while in cardiac combined 145- and 135-kDa band intensity decreased (P < 0.05). Treatment increased skeletal (P < 0.01) but not cardiac calpastatin mRNA steady-state levels. Three types of cardiac calpastatin mRNA transcripts were identified by 5'-RACE. Types I and II encoded a putative XL region that originated either from exon 1x(A) or exon 1x(B), arranged in tandem. Type III predominated in skeletal muscle and originated from exon 1u, which was located 40-50 kb 3' to exons 1x(A) and 1x(B). The region 5' to exon 1u may act as an independent promoter regulated by a cAMP-dependent mechanisms, thereby explaining the differential response of calpastatin to adrenergic stimulation in cardiac and skeletal muscle. Copyright 2001 Academic Press. |
