| First Author | Tsutsumi K | Year | 2008 |
| Journal | Genes Cells | Volume | 13 |
| Issue | 9 | Pages | 949-64 |
| PubMed ID | 18691334 | Mgi Jnum | J:142139 |
| Mgi Id | MGI:3820458 | Doi | 10.1111/j.1365-2443.2008.01219.x |
| Citation | Tsutsumi K, et al. (2008) Palmitoylation-dependent endosomal localization of AATYK1A and its interaction with Src. Genes Cells 13(9):949-64 |
| abstractText | Apoptosis-associated tyrosine kinase 1 (AATYK1), also named LMTK1, was previously isolated as an apoptosis-related gene from 32Dcl3 myeloid precursor cells, but its precise function remains unknown. AATYK1A, an isoform without a transmembrane domain, is highly expressed in neurons. We identified palmitoylation of AATYK1A at three N-terminal cysteine residues in cortical cultured neurons and COS-7 cells and found that palmitoylation determined localization of AATYK1A to the transferrin receptor-positive recycling endosomes. Further, we identified the tyrosine kinase Src as a novel AATYK1A-interacting protein. Src and Fyn phosphorylated AATYK1A at tyrosines 25 and 46 in a palmitoylation-dependent manner. The association of AATYK1A with Src in endosomes was also found to be palmitoylation-dependent. These results indicate that palmitoylation is a critical factor not only for the subcellular localization of AATYK1A but also for its interaction with Src. |
