| First Author | Sedmak T | Year | 2010 |
| Journal | J Cell Biol | Volume | 189 |
| Issue | 1 | Pages | 171-86 |
| PubMed ID | 20368623 | Mgi Jnum | J:161660 |
| Mgi Id | MGI:4460838 | Doi | 10.1083/jcb.200911095 |
| Citation | Sedmak T, et al. (2010) Intraflagellar transport molecules in ciliary and nonciliary cells of the retina. J Cell Biol 189(1):171-86 |
| abstractText | The assembly and maintenance of cilia require intraflagellar transport (IFT), a process mediated by molecular motors and IFT particles. Although IFT is a focus of current intense research, the spatial distribution of individual IFT proteins remains elusive. In this study, we analyzed the subcellular localization of IFT proteins in retinal cells by high resolution immunofluorescence and immunoelectron microscopy. We report that IFT proteins are differentially localized in subcompartments of photoreceptor cilia and in defined periciliary target domains for cytoplasmic transport, where they are associated with transport vesicles. IFT20 is not in the IFT core complex in photoreceptor cilia but accompanies Golgi-based sorting and vesicle trafficking of ciliary cargo. Moreover, we identify a nonciliary IFT system containing a subset of IFT proteins in dendrites of retinal neurons. Collectively, we provide evidence to implicate the differential composition of IFT systems in cells with and without primary cilia, thereby supporting new functions for IFT beyond its well-established role in cilia. |
