| First Author | Li H | Year | 2000 |
| Journal | Genomics | Volume | 67 |
| Issue | 3 | Pages | 284-90 |
| PubMed ID | 10936050 | Mgi Jnum | J:63863 |
| Mgi Id | MGI:1861869 | Doi | 10.1006/geno.2000.6269 |
| Citation | Li H, et al. (2000) The cloning and nucleotide sequence of human ST2L cDNA. Genomics 67(3):284-90 |
| abstractText | The ST2 gene is a member of the IL-1 receptor family and is hypothesized to be involved in helper T cell function, but its functional ligand and physiological role remain unknown. We have cloned the human ST2L cDNA that encodes a distinct type of membrane-bound ST2 protein. The predicted 556-amino-acid sequence showed 67% identity to the mouse ST2L protein. The human ST2 gene (IL1RL1) contains 13 exons and spans 40 kb in length. Its exon-intron organization was elucidated from a registered human genomic sequence derived from chromosome 2q, which contains three other genes belonging to the IL-1 receptor family in an approximately 202-kb genomic region. The tissue distribution of ST2 expression was examined by RT-PCR, and the soluble form (ST2, IL1RL1-a) and ST2L (IL1RL1-b) appear to be expressed differentially. We also established stable transfectants of a human glioblastoma cell line, T98G, that express human ST2L constitutively, and we confirmed cell-surface expression of human ST2L protein on the transfectants. Copyright 2000 Academic Press. |
