| First Author | Lee KH | Year | 2004 |
| Journal | FEBS Lett | Volume | 578 |
| Issue | 1-2 | Pages | 47-52 |
| PubMed ID | 15581614 | Mgi Jnum | J:94273 |
| Mgi Id | MGI:3511730 | Doi | 10.1016/j.febslet.2004.10.076 |
| Citation | Lee KH, et al. (2004) NMDA receptor-mediated calcium influx plays an essential role in myoblast fusion. FEBS Lett 578(1-2):47-52 |
| abstractText | Ca2+ influx is known to be prerequisite for myoblast fusion during skeletal muscle differentiation. Here, we show that the N-methyl-D-aspartate (NMDA) receptor is involved in the Ca2+ influx of C2C12 myoblasts. NMDA receptor (NR) 1 and NR2D were expressed in the myoblasts during muscle differentiation. Using Ca2+ imaging analysis, Ca2+ influx through NRs was directly measured at a single-cell level. l-Glutamate increased myoblast fusion as well as intracellular Ca2+ levels, and both effects were completely blocked by MK801, a selective antagonist of NRs. Furthermore, treatment with the Ca2+ ionophore A23187 recovered MK801-mediated inhibition of myoblast fusion. These results suggest that the NRs may play an important role in myoblast fusion by mediating Ca2+ influx. |
