| First Author | Bouissou A | Year | 2014 |
| Journal | EMBO J | Volume | 33 |
| Issue | 2 | Pages | 114-28 |
| PubMed ID | 24421324 | Mgi Jnum | J:206452 |
| Mgi Id | MGI:5550304 | Doi | 10.1002/embj.201385967 |
| Citation | Bouissou A, et al. (2014) gamma-Tubulin Ring Complexes and EB1 play antagonistic roles in microtubule dynamics and spindle positioning. EMBO J 33(2):114-28 |
| abstractText | gamma-Tubulin is critical for microtubule (MT) assembly and organization. In metazoa, this protein acts in multiprotein complexes called gamma-Tubulin Ring Complexes (gamma-TuRCs). While the subunits that constitute gamma-Tubulin Small Complexes (gamma-TuSCs), the core of the MT nucleation machinery, are essential, mutation of gamma-TuRC-specific proteins in Drosophila causes sterility and morphological abnormalities via hitherto unidentified mechanisms. Here, we demonstrate a role of gamma-TuRCs in controlling spindle orientation independent of MT nucleation activity, both in cultured cells and in vivo, and examine a potential function for gamma-TuRCs on astral MTs. gamma-TuRCs locate along the length of astral MTs, and depletion of gamma-TuRC-specific proteins increases MT dynamics and causes the plus-end tracking protein EB1 to redistribute along MTs. Moreover, suppression of MT dynamics through drug treatment or EB1 down-regulation rescues spindle orientation defects induced by gamma-TuRC depletion. Therefore, we propose a role for gamma-TuRCs in regulating spindle positioning by controlling the stability of astral MTs. |
