| First Author | Lee KW | Year | 2013 |
| Journal | Biochem Biophys Res Commun | Volume | 435 |
| Issue | 2 | Pages | 239-43 |
| PubMed ID | 23643813 | Mgi Jnum | J:201850 |
| Mgi Id | MGI:5515864 | Doi | 10.1016/j.bbrc.2013.04.072 |
| Citation | Lee KW, et al. (2013) F-box only protein 9 is required for adipocyte differentiation. Biochem Biophys Res Commun 435(2):239-43 |
| abstractText | The objective of this study is to investigate whether F-box only protein 9 (FBXO9), an ubiquitination E3 ligase, has a functional role in adipocyte differentiation. Expression of FBXO9 was compared between obese mice and control lean mice using real-time PCR. Also, expression pattern of FBXO9 was monitored during 3T3-L1 adipocyte differentiation. FBXO9 was highly expressed in obese mice, and increased in the early stages of adipogenesis. To verify a functional role of FBXO9 in adipogenesis, FBXO9 was knocked down using transfection of siRNAs against FBXO9 into 3T3-L1 cells during the induction of adipogenesis. Knockdown of FBXO9 in early stage of adipogenesis almost completely inhibited adipogenesis, and CCAAT/enhancer binding protein beta (C/EBPbeta) levels were significantly reduced. However, the cells stably expressing C/EBPbeta were fairly differentiated into adipocytes in the FBXO9 knockdown condition. These results suggest that FBXO9 is required for adipocyte differentiation, and C/EBPbeta plays a role in the effect of FBXO9 on adipogenesis. |
