| First Author | Choi YJ | Year | 2009 |
| Journal | FEBS Lett | Volume | 583 |
| Issue | 8 | Pages | 1292-8 |
| PubMed ID | 19303412 | Mgi Jnum | J:147987 |
| Mgi Id | MGI:3843123 | Doi | 10.1016/j.febslet.2009.03.023 |
| Citation | Choi YJ, et al. (2009) Nm23-M5 mediates round and elongated spermatid survival by regulating GPX-5 levels. FEBS Lett 583(8):1292-8 |
| abstractText | Nucleoside diphosphate (NDP) kinases are involved in numerous regulatory processes associated with proliferation, development, and differentiation. Previously, we cloned a new member of the NDPK family from mouse, Nm23-M5, which encodes a 211-amino acid protein and has 86% identity to the human Nm23-H5 [Hwang, K.C., Ok, D.W., Hong, J.C., Kim, M.O. and Kim, J.H. (2003) Cloning, sequencing, and characterization of the murine Nm23-M5 gene during mouse spermatogenesis and spermiogenesis. Biochem. Biophys. Res. Commun. 306, 198-207]. To better understand Nm23-M5 function, we generated transgenic mice with reduced Nm23-M5 levels in vivo using a short hairpin RNA (shRNA) knock-down system. Nm23-M5 expression was markedly reduced, as indicated by Northern and Western blot analysis. Nm23-M5 shRNA transgenic mice exhibited reduced numbers of haploid cells. Furthermore, the antioxidant enzyme glutathione peroxidase 5 (GPX-5) is regulated by Nm23-M5 at the level of both expression and activity. These results reveal that expression of Nm23-M5 plays a critical role in spermiogenesis by increasing the cellular levels of GPX-5 to eliminate reactive oxygen species. |
