| First Author | Wyss A | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 271 |
| Issue | 2 | Pages | 334-6 |
| PubMed ID | 10799297 | Mgi Jnum | J:66309 |
| Mgi Id | MGI:1928253 | Doi | 10.1006/bbrc.2000.2619 |
| Citation | Wyss A, et al. (2000) Cloning and expression of beta,beta-carotene 15,15'-dioxygenase. Biochem Biophys Res Commun 271(2):334-6 |
| abstractText | beta,beta-Carotene 15,15'-dioxygenase cleaves beta-carotene into two molecules of retinal and is therefore the key enzyme in beta-carotene metabolism to vitamin A. In the present study, it was possible to enrich the chicken beta,beta-carotene 15,15'-dioxygenase to such an extent that partial amino acid sequence information could be obtained to design degenerate oligonucleotides. With RT-PCR a cDNA fragment could be obtained and used subsequently in a radioactive screening of a chicken duodenal expression library. We cloned the first eukaryotic beta,beta-carotene 15,15'-dioxygenase which symmetrically cleaves beta-carotene at the 15,15'-double bond. Copyright 2000 Academic Press. |
