| First Author | Fundele R | Year | 1987 |
| Journal | Dev Biol | Volume | 124 |
| Issue | 2 | Pages | 562-6 |
| PubMed ID | 2824255 | Mgi Jnum | J:203034 |
| Mgi Id | MGI:5523880 | Doi | 10.1016/0012-1606(87)90510-0 |
| Citation | Fundele R, et al. (1987) Developmental activation of phosphoglycerate mutase-2 in the testis of the mouse. Dev Biol 124(2):562-6 |
| abstractText | The expression of the phosphoglycerate mutase locus Pgam-2 which synthesizes the muscle-specific PGAM-B subunit was analyzed in the testis of the mouse. No PGAM-B activity was detected in testes of newborn mice, in which only the PGAM-AA isozyme was observed. PGAM-B was first observed between Day 14 and Day 16 of postnatal development. In adult males approximately 50% of total PGAM activity is contributed by the PGAM-B subunit and 50% by the PGAM-A subunit. Immunohistochemical studies show that in the testis PGAM-B is localized exclusively in germ cells. PGAM-B is detected in pachytene spermatocytes and in spermatids, but not in earlier stages of spermatogenesis. The muscle-specific PGAM isozyme was also found in testes of bull, cat, and rat, as well as in human sperm. PGAM-B might thus be useful as a marker for germ cell differentiation, along with other germ cell-specific proteins. |
