| First Author | Vacher J | Year | 1992 |
| Journal | Mol Cell Biol | Volume | 12 |
| Issue | 2 | Pages | 856-64 |
| PubMed ID | 1370712 | Mgi Jnum | J:754 |
| Mgi Id | MGI:49288 | Doi | 10.1128/mcb.12.2.856 |
| Citation | Vacher J, et al. (1992) raf regulates the postnatal repression of the mouse alpha-fetoprotein gene at the posttranscriptional level. Mol Cell Biol 12(2):856-64 |
| abstractText | The mouse alpha-fetoprotein (AFP) gene is transcribed at a high rate in liver during the second half of gestation. Its steady-state mRNA levels decrease 10(4)-fold shortly after birth, at least in part as the consequence of a dramatic decrease in its transcription rate. The final basal level of AFP mRNA in adult liver is influenced by a trans-acting locus on chromosome 15 termed raf. Two strategies were used to demonstrate that the raf gene acts posttranscriptionally to affect the processing and/or stability of AFP transcripts. Transgenic mouse studies demonstrated that raf gene action is independent of both positive and negative transcription control elements of the AFP gene. Nuclear run-on analysis was used to confirm that transcriptions of both AFP transgenes and another endogenous raf-responsive gene, H19, are invariant with respect to the raf genotype. Thus, the postnatal repression of the AFP gene is mediated by both transcriptional and posttranscriptional mechanisms. |
