| First Author | Wood L | Year | 1991 |
| Journal | Gene | Volume | 98 |
| Issue | 2 | Pages | 249-52 |
| PubMed ID | 2016065 | Mgi Jnum | J:23858 |
| Mgi Id | MGI:71746 | Doi | 10.1016/0378-1119(91)90181-a |
| Citation | Wood L, et al. (1991) Isolation of a mouse genomic clone containing four tRNACys-encoding genes. Gene 98(2):249-52 |
| abstractText | A cluster of four tRNACys-encoding genes with the anticodon GCA was found on a murine genomic clone containing an 18-kb DNA insert. Three of the four genes encode the identical tRNA, whereas the fourth gene has an altered nucleotide (nt) sequence. Two of the genes within a 2-kb PvuII fragment have the same polarity and are separated by only 921 bp. These two tRNAs have a different primary sequence. The changes in the nt sequences occur within three stems of the tRNA cloverleaf structure and weaken the strength of the H-bonds within the stems. All four genes (designated i-iv) have the 3' structural element that has been proposed as the transcription termination signal [Bogenhagen and Brown, Cell 24 (1981) 261-270]. The remainder of the flanking regions of the three identical tRNAs are very similar to each other, whereas the flanking regions of the fourth tRNA are distinctly different. |
