| First Author | Senda T | Year | 1996 |
| Journal | Biochem Biophys Res Commun | Volume | 223 |
| Issue | 2 | Pages | 329-34 |
| PubMed ID | 8670282 | Mgi Jnum | J:33616 |
| Mgi Id | MGI:81094 | Doi | 10.1006/bbrc.1996.0894 |
| Citation | Senda T, et al. (1996) The tumor suppressor protein APC colocalizes with beta-catenin in the colon epithelial cells. Biochem Biophys Res Commun 223(2):329-34 |
| abstractText | The APC gene is mutated in familial adenomatous polyposis and sporadic colorectal tumors. The product of this gene is a 300 kDa cytoplasmic protein associated with catenin. In the present study, we examined the subcellular localization of the APC protein and beta-catenin in the mouse colon by double-labeling immunocytochemistry. While the APC protein was localized in the lateral and apical cytoplasm and in microvilli of the epithelial cells, beta-catenin was present exclusively in the lateral cytoplasm. Double-labeling-immunoelectron microscopy demonstrated precise colocalization of the APC protein and beta-catenin along the lateral plasma membrane. These results suggest that the APC protein functions in cooperation with beta-catenin in the lateral cytoplasm but has other functions independent of beta-catenin in the apical cytoplasm and in microvilli. |
