| First Author | Hitsumoto Y | Year | 1996 |
| Journal | Immunology | Volume | 89 |
| Issue | 2 | Pages | 200-4 |
| PubMed ID | 8943715 | Mgi Jnum | J:125137 |
| Mgi Id | MGI:3757616 | Doi | 10.1046/j.1365-2567.1996.d01-741.x |
| Citation | Hitsumoto Y, et al. (1996) Enhancement of CD3-mediated thymocyte apoptosis by the cross-linkage of heat-stable antigen. Immunology 89(2):200-4 |
| abstractText | Heat-stable antigen (HSA) is a murine differentiating antigen that is expressed on both CD4-CD8- double-negative and CD4+CD8+ double-positive thymocytes but not CD4+ or CD8+ single-positive thymocytes. Effects of anti-HSA monoclonal antibody, R13, on thymocyte apoptosis induced by various stimulations were investigated by a single-cell suspension culture system. Immobilized R13 enhanced the CD3-mediated DNA fragmentation and killing of thymocytes but not the dexamethasone-induced or phorbol myristate acetate-induced killing of thymocytes. Immobilized R13 by itself could not induce thymocyte apoptosis. Soluble R13 enhanced CD3-mediated apoptosis when HSA and T-cell receptor (TCR)/CD3 were co-cross-linked by a cross-reactive secondary antibody. Even without the cross-reactive secondary antibody, soluble R13 enhanced CD3-mediated apoptosis, although a greater than 100-fold increase in the amount of R13 was needed to give a similar enhancement compared with immobilized R13. Neither R13 by itself nor R13 plus secondary antibody induced cytosolic calcium influx, whereas R13 enhanced CD3-mediated cytosolic calcium increase. These results suggest a functional role of HSA in promoting the activation-induced apoptosis of thymocytes and the involvement of HSA in negative selection. |
