| First Author | Bhat NK | Year | 1984 |
| Journal | Biochem Biophys Res Commun | Volume | 118 |
| Issue | 2 | Pages | 514-22 |
| PubMed ID | 6200105 | Mgi Jnum | J:144770 |
| Mgi Id | MGI:3831659 | Doi | 10.1016/0006-291x(84)91333-0 |
| Citation | Bhat NK, et al. (1984) The transport and processing of carbamyl phosphate synthetase-I in mouse hepatic mitochondria. Biochem Biophys Res Commun 118(2):514-22 |
| abstractText | Carbamyl phosphate synthetase-I (CPS-I)2, purified from mouse hepatic mitochondria consists of electrophoretically homogeneous polypeptide species of 160 kilodaltons molecular weight (Kd). Monospecific antibody to CPS-I immunoprecipitated a putative precursor of 165Kd protein from in vitro translation products programmed with mouse liver free polysomes or poly(A) RNA. Isolated mitochondrial particles can take up and process pCPS-I into mature CPS-I of 160Kd in an in vitro transport system. The in vitro transport of CPS-I is energy dependent and requires intact mitochondria. The processing of pCPS-I appears to involve a single endoproteolytic cleavage. |
