| First Author | Ronai A | Year | 1993 |
| Journal | Biochem Genet | Volume | 31 |
| Issue | 7-8 | Pages | 279-94 |
| PubMed ID | 8274135 | Mgi Jnum | J:15451 |
| Mgi Id | MGI:63572 | Citation | Ronai A, et al. (1993) Esterase-30 (ES-30) of the house mouse: biochemical characterization and genetics of a new carboxylesterase isozyme linked to cluster-2 loci on chromosome 8. Biochem Genet 31(7-8):279-94 |
| abstractText | A new carboxylesterase isozyme (EC 3.1.1.1), designated ES-30, is described in mouse liver. Two phenotypes were distinguished, ES-30A, a possible null type, was found in SPE/Pas and in other lines derived from Mus spretus, and ES-30B was found in BALB/cJ and other laboratory inbred strains. ES-30B is characterized by a distinct electrophoretic band when stained using 5-bromoindoxyl acetate as the substrate. After isolation and purification from other esterases by ion-exchange chromatography and molecular sieving, the molecular mass was estimated by two independent methods to be 62 and 64 kDa, respectively. The activity of ES-30B is higher in adult males than in females and can be stimulated in vivo by testosterone. The distribution of phenotypes on the progeny of a backcross series suggests a separate locus, Es-30, with the allele a for absence and b for presence of the isozyme. Locus Es-30 is shown to be closely linked to Es-2 and to Es-7 of cluster-2 on chromosome 8. The gene order Es-9--Got-2--(Es-2, Es-7, Es-30) is suggested. |
