| First Author | Petit FG | Year | 2004 |
| Journal | Mech Ageing Dev | Volume | 125 |
| Issue | 10-11 | Pages | 719-32 |
| PubMed ID | 15541767 | Mgi Jnum | J:93835 |
| Mgi Id | MGI:3505823 | Doi | 10.1016/j.mad.2004.03.009 |
| Citation | Petit FG, et al. (2004) The regulation of COUP-TFII gene expression by Ets-1 is enhanced by the steroid receptor co-activators. Mech Ageing Dev 125(10-11):719-32 |
| abstractText | Recent phenotypic analysis of orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) [NR2F2] knockout mice shows that COUP-TFII is involved in the angiogenic process in the developing embryos. Since Ets-1 expression is also correlated with angiogenesis, and both Ets-1 and COUP-TFII mRNA are present in mesenchymal cells, we have sought to determine whether Ets-1 is a potential regulator of COUP-TFII gene expression. For this purpose, we performed transient transfection experiments using a luciferase reporter construct containing the mouse COUP-TFII promoter. We found that the COUP-TFII promoter activity is indeed regulated by Ets-1. We have identified two identical inverted potential ETS-binding sites located 47 nucleotides downstream of the start site. Mutation of both sites reduced the ability of Ets-1 to enhance the COUP-TFII promoter activity. Furthermore, other members of the ETS family such as Ets-2 or ETV1 are also potent regulators of the COUP-TFII promoter. Finally, the induction of the COUP-TFII gene is strongly enhanced by the expression of steroid receptor co-activator factors through a direct interaction with Ets-1. These results indicate that COUP-TFII is a potential downstream target of Ets-1 and it may partially mediate the Ets-1 function in angiogenesis. |
