| First Author | Tsuzuki K | Year | 1994 |
| Journal | Brain Res Mol Brain Res | Volume | 26 |
| Issue | 1-2 | Pages | 37-46 |
| PubMed ID | 7531804 | Mgi Jnum | J:21285 |
| Mgi Id | MGI:69304 | Doi | 10.1016/0169-328x(94)90071-x |
| Citation | Tsuzuki K, et al. (1994) Ion permeation properties of the cloned mouse epsilon 2/zeta 1 NMDA receptor channel. Brain Res Mol Brain Res 26(1-2):37-46 |
| abstractText | The heteromeric mouse epsilon 2/zeta 1 N-methyl-D-aspartate (NMDA) receptor was expressed in Xenopus oocytes, and its channel properties were studied using both the outside-out patch-clamp and two-microelectrode voltage-clamp techniques. In the cloned receptor channel, permeation properties of monovalent and divalent cations, and voltage-dependent block by Mg2+ were similar to those reported previously in the native NMDA receptor channels. The sequence of single-channel conductances for alkali metals was Rb+ > Cs+ approximately K+ > Na+ > Li+, whereas the sequence of relative permeabilities was Cs+ > Rb+ > K+ approximately Na+ > Li+. The single-channel conductances measured in isotonic Ca2+, Sr2+ and Ba2+ solutions were almost equal, and approximately one-fifth of the value in the Na+ solution, although the permeabilities for these alkaline earth cations were higher than for Na+. It is likely that Ca2+, Sr2+ and Ba2+ would enter the NMDA receptor channel more easily than Na+, but would bind to a site in the channel more tightly, the net effect being a reduced value of the current. |
