| First Author | Mohamed OA | Year | 2001 |
| Journal | Dev Biol | Volume | 229 |
| Issue | 1 | Pages | 237-49 |
| PubMed ID | 11133167 | Mgi Jnum | J:66810 |
| Mgi Id | MGI:1929308 | Doi | 10.1006/dbio.2000.9942 |
| Citation | Mohamed OA, et al. (2001) High-mobility group proteins 14 and 17 maintain the timing of early embryonic development in the mouse. Dev Biol 229(1):237-49 |
| abstractText | The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. We report that both mRNA species and both proteins are present throughout oogenesis and preimplantation development of the mouse. When antisense oligonucleotides targeting each mRNA species are injected into one-cell embryos, the proteins become depleted at the two- and four-cell stages and reaccumulate at the eight-cell stage. One-cell embryos injected with antisense oligonucleotides targeting both HMG-14 and HMG-17 cleave to the two-cell stage. Subsequent cleavages, however, are delayed compared with control-injected embryos. Nevertheless, these embryos ultimately reach the blastocyst stage. Similarly, injection into the nuclei of two-cell embryos of a peptide corresponding to the common nucleosome-binding domain of HMG-14 and HMG-17 delays progression to the four-cell stage. Furthermore, both RNA and protein synthesis is transiently reduced in antisense-injected embryos compared with injected controls. These results identify HMG-14 and HMG-17 as constitutive components of mouse oocyte and embryonic chromatin and establish a link between the structure of embryonic chromatin and the normal progression of embryonic development. |
