| First Author | Pitera JE | Year | 1999 |
| Journal | Gastroenterology | Volume | 117 |
| Issue | 6 | Pages | 1339-51 |
| PubMed ID | 10579975 | Mgi Jnum | J:60124 |
| Mgi Id | MGI:1352790 | Doi | 10.1016/s0016-5085(99)70284-2 |
| Citation | Pitera JE, et al. (1999) Coordinated expression of 3' hox genes during murine embryonal gut development: an enteric Hox code. Gastroenterology 117(6):1339-51 |
| abstractText | BACKGROUND & AIMS: Hox genes are highly conserved developmental control genes that may be organized and expressed in the form of a code required for correct morphogenesis. Little is known about their control of the embryonal gut. However, Hox paralogues 4 and 5, which are expressed at the sites of origin of vagal neural crest cells and splanchnic mesoderm, are likely to be important. We have studied the expression domains of these genes in the gut both spatially and temporally. METHODS: CD1 mice embryos of embryonic days E8.5-E17.5 were studied. The spatial and temporal expression patterns of messenger RNA of Hoxa4, b4, c4, d4, a5, c5, and b5 homeoprotein were determined by in situ hybridization and immunohistochemistry in whole embryos, whole gastrointestinal tracts, and vibratome sections. RESULTS: There were different spatial, temporal, and combinatorial expression patterns in different morphological regions: foregut, prececal gut, cecum, and postcecal gut. Two dynamic gradients, rostral and caudal, were coordinated with nested expression domains along the gut primordium. Region-specific domains were present in the stomach and cecum. CONCLUSIONS: The expression patterns of genes in paralogous groups 4 and 5 suggest that they are organized to form a specific enteric Hox code required for correct enteric development. |
