| First Author | Roldán-Sastre A | Year | 2021 |
| Journal | Front Neuroanat | Volume | 15 |
| Pages | 686279 | PubMed ID | 34248508 |
| Mgi Jnum | J:347302 | Mgi Id | MGI:7622106 |
| Doi | 10.3389/fnana.2021.686279 | Citation | Roldan-Sastre A, et al. (2021) Cellular Diversity and Differential Subcellular Localization of the G-Protein G(alphao) Subunit in the Mouse Cerebellum. Front Neuroanat 15:686279 |
| abstractText | Heterotrimeric guanine nucleotide-binding proteins (G proteins) transduce signals from G protein-coupled receptors (GPCRs) to effector ion channels and enzymes G(alphao), a member of the pertussis toxin-sensitive G (i/o) family, is widely expressed in the brain, although its role within a neuronal context remains largely unknown. Using immunohistochemical and quantitative immunoelectron microscopy techniques, we have investigated the expression, cellular and subcellular localization of G(alphao) in the cerebellar cortex. Histoblot revealed that G(alphao) is expressed in many brain regions, including the cerebellum. At the cellular level, G(alphao) protein was distributed in Purkinje cells, basket cells, stellate cells, granule cells and Golgi cells. At the subcellular level, pre-embedding immunoelectron microscopy revealed mainly a postsynaptic localization of G(alphao) along the extrasynaptic plasma membrane of Purkinje cell dendritic shafts and spines, and dendrites of basket, stellate and granule cells. To a lesser extent, immunolabeling for G(alphao) was localized in different types of axon terminals establishing excitatory synapses. Moreover, post-embedding immunoelectron microscopy revealed the synaptic localization of G(alphao) on PSDs of glutamatergic synapses between Purkinje cell spines and parallel fiber terminals and its co-localization with GABA (B1) in the same spines. Quantitative analysis of G(alphao) immunoparticles revealed they preferentially localized on the cytoplasmic face of the plasma membrane. Furthermore, the analysis revealed a high concentration of G(alphao) around excitatory synapses on Purkinje cell dendritic spines, but a uniform distribution in granule cell dendrites. These molecular-anatomical findings suggest that G(alphao) is a major signal transducer of specific GPCRs in different neuronal populations in the cerebellum. |
