| First Author | Esaki N | Year | 1981 |
| Journal | Biochemistry | Volume | 20 |
| Issue | 15 | Pages | 4492-6 |
| PubMed ID | 6456763 | Mgi Jnum | J:387219 |
| Mgi Id | MGI:8351854 | Doi | 10.1021/bi00518a039 |
| Citation | Esaki N, et al. (1981) Enzymatic synthesis of selenocysteine in rat liver. Biochemistry 20(15):4492-6 |
| abstractText | We have investigated selenocysteine (2-amino-3-hydroselenopropionic acid) synthesis with cystathionine beta-synthase (EC 4.2.1.22) and cystathionine gamma-lyase (EC 4.4.1.1) of rat liver. When selenohomocysteine and serine were incubated with cystathionine beta-synthase, selenocystathionine was formed at a rate of 69% of that of cystathionine synthesis. Cystathionine gamma-lyase catalyzed alpha, gamma elimination of selenocystathionine to yield alpha-ketobutyrate, selenocysteine, and NH3. The reaction rate was about 3 times higher than that of cystathionine elimination. Cystathionine beta-synthase, however, did not catalyze direct formation of selenocysteine from serine and H2Se. Thus, selenocysteine is synthesized from selenohomocysteine and cystathionine beta-synthase and cystathionine gamma-lyase reactions. We confirmed this synthetic pathway also with a mixture of both enzymes and with a homogenate of rat liver. |
