| First Author | Robbins D | Year | 2012 |
| Journal | FEBS Lett | Volume | 586 |
| Issue | 23 | Pages | 4108-13 |
| PubMed ID | 23123091 | Mgi Jnum | J:191729 |
| Mgi Id | MGI:5462485 | Doi | 10.1016/j.febslet.2012.10.036 |
| Citation | Robbins D, et al. (2012) Involvement of PTEN in TPA-mediated p53-activation in mouse skin epidermal JB6 cells. FEBS Lett 586(23):4108-13 |
| abstractText | Our recent studies suggest a unique role of p53 during the early stage of cancer development. However, how p53 activation is regulated during TPA treatment remains elusive. We used murine skin epidermal JB6 promotion-sensitive (P+) and promotion resistant (P-) cells to observe differential expression of PTEN during TPA-induced p53 activation. Total PTEN expression was decreased in only P+ cells. Nuclear expression of PTEN increased and complex formation between PTEN and p53 occurred in P+ cells treated with TPA. Knocking down PTEN expression via siRNA inhibited TPA-induced Bax expression. Similar effects were seen with the p53 inhibitor, pifithrin-alpha. Cells that were transfected with siRNA to PTEN exhibited enhanced tumorigenicity. Our findings suggest PTEN mediates TPA-induced p53 activation. |
