| First Author | Schéele S | Year | 2006 |
| Journal | Matrix Biol | Volume | 25 |
| Issue | 5 | Pages | 301-5 |
| PubMed ID | 16631359 | Mgi Jnum | J:159151 |
| Mgi Id | MGI:4441265 | Doi | 10.1016/j.matbio.2006.03.004 |
| Citation | Scheele S, et al. (2006) Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain. Matrix Biol 25(5):301-5 |
| abstractText | We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-alpha1beta1gamma1 (laminin-111), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and immunostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha1 chain. AL-4 has also been marketed as an alpha1 chain specific probe, but we show here that AL-4 detects mouse laminin beta1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross-region, but seems to primarily react with laminin beta1 chain. |
