| First Author | Kasai K | Year | 2012 |
| Journal | FEBS Lett | Volume | 586 |
| Issue | 4 | Pages | 325-9 |
| PubMed ID | 22285492 | Mgi Jnum | J:201297 |
| Mgi Id | MGI:5512942 | Doi | 10.1016/j.febslet.2012.01.012 |
| Citation | Kasai K, et al. (2012) Novel assay with fluorescence-labelled PrP peptides for differentiating L-type atypical and classical BSEs, and scrapie. FEBS Lett 586(4):325-9 |
| abstractText | Characteristic differences of prions may account for the conformational diversity of the pathogenic isoform of prion protein (PrP(Sc)). Here, we applied a protein detection procedure by using fluorescent-labelled peptides for detecting PrP(Sc). Five prion protein (PrP) related peptides were found to change significantly their fluorescent intensities with prion-affected animal samples. Their reactivity was different among atypical L-BSE, classical BSE and scrapie. The pull-down assay revealed that they precipitated PrP(Sc) specifically. These findings suggest that fluorescent intensity changes depend on peptide-PrP(Sc) binding. This novel approach may distinguish the fine structural differences in PrP(Sc), which were not detected by the pull-down assay. |
