| First Author | Carbone M | Year | 2006 |
| Journal | J Mol Biol | Volume | 363 |
| Issue | 4 | Pages | 773-85 |
| PubMed ID | 16979186 | Mgi Jnum | J:201316 |
| Mgi Id | MGI:5512961 | Doi | 10.1016/j.jmb.2006.05.077 |
| Citation | Carbone M, et al. (2006) PARP-1 interaction with VP1 capsid protein regulates polyomavirus early gene expression. J Mol Biol 363(4):773-85 |
| abstractText | Poly(ADP-ribose)polymerases are involved in fundamental cellular events as well as they seem to be associated to some viral infection process. In this work, the poly(ADP-ribose)polymerase-1 (PARP-1) role in the polyomavirus life cycle has been investigated. Early viral transcription was reduced by competitive inhibitors of PARPs in Swiss 3T3 cells and almost abolished in PARP-1 knockout fibroblasts and in wild-type fibroblasts when PARP-1 was silenced by RNA interference. In vivo chromatin immunoprecipitation assays showed that poly(ADP-ribosyl)ation (poly(ADP-ribose)) facilitates the release of the capsid protein viral protein 1 (VP1) from the chromatin of infecting virions. In vitro experiments demonstrated that VP1 stimulates the enzymatic activity of PARP-1 and binds non-covalently both protein-free and PARP-1-bound poly(ADP-ribose). Our studies suggest that PARP-1 promotes the complete VP1 displacement from viral DNA favouring the viral early transcription. |
