| First Author | Williams JM | Year | 2000 |
| Journal | Brain Res Mol Brain Res | Volume | 77 |
| Issue | 2 | Pages | 258-66 |
| PubMed ID | 10837920 | Mgi Jnum | J:215663 |
| Mgi Id | MGI:5605961 | Doi | 10.1016/s0169-328x(00)00061-9 |
| Citation | Williams JM, et al. (2000) Sequential increase in Egr-1 and AP-1 DNA binding activity in the dentate gyrus following the induction of long-term potentiation. Brain Res Mol Brain Res 77(2):258-66 |
| abstractText | Establishment of long-term potentiation (LTP) at perforant path synapses is highly correlated with increased expression of Egr and AP-1 transcription factors in rat dentate gyrus granule cells. We have investigated whether increased transcription factor levels are reflected in increased transcription factor activity by assessing Egr and AP-1 DNA binding activity using gel shift assays. LTP produced an increase in binding to the Egr element, which was NMDA receptor-dependent and correlated closely with our previously reported increase in Egr-1 (zif/268) protein levels. Supershift analysis confirmed involvement of Egr-1, but not Egr-2 in the DNA binding activity. AP-1 DNA binding was also rapidly elevated in parallel with protein levels, however, the peak increase in activity was delayed until 4 h, a time point when we have previously shown that only jun-D protein was elevated. These data indicate that binding of Egr-1 and AP-1 to their response elements is increased in two phases. This may result in activation of distinct banks of target genes which contribute to the establishment of persistent LTP. |
