| First Author | Westergaard UB | Year | 2003 |
| Journal | Eur J Biochem | Volume | 270 |
| Issue | 8 | Pages | 1850-4 |
| PubMed ID | 12694198 | Mgi Jnum | J:190000 |
| Mgi Id | MGI:5447625 | Doi | 10.1046/j.1432-1033.2003.03549.x |
| Citation | Westergaard UB, et al. (2003) Tetranectin binds hepatocyte growth factor and tissue-type plasminogen activator. Eur J Biochem 270(8):1850-4 |
| abstractText | In the search for new ligands for the plasminogen kringle 4 binding-protein tetranectin, it has been found by ligand blot analysis and ELISA that tetranectin specifically bound to the plasminogen-like hepatocyte growth factor and tissue-type plasminogen activator. The dissociation constants of these complexes were found to be within the same order of magnitude as the one for the plasminogen-tetranectin complex. The study also revealed that tetranectin did not interact with the kindred proteins: macrophage-stimulating protein, urokinase-type plasminogen activator and prothrombin. In order to examine the function of tetranectin, a kinetic analysis of the tPA-catalysed plasminogen activation was performed. The kinetic parameters of the tetranectin-stimulated enhancement of tPA were comparable to fibrinogen fragments, which are so far the best inducer of tPA-catalysed plasminogen activation. The enhanced activation was suggested to be caused by tetranectin's ability to bind and accumulate tPA in an active conformation. |
