| First Author | Zafarana G | Year | 2000 |
| Journal | EMBO J | Volume | 19 |
| Issue | 21 | Pages | 5856-63 |
| PubMed ID | 11060036 | Mgi Jnum | J:65668 |
| Mgi Id | MGI:1927030 | Doi | 10.1093/emboj/19.21.5856 |
| Citation | Zafarana G, et al. (2000) Erythroid overexpression of C/EBPgamma in transgenic mice affects gamma-globin expression and fetal liver erythropoiesis. EMBO J 19(21):5856-63 |
| abstractText | The CCAAT boxes of the beta-like globin genes interact with three proteins: NF-Y, GATA-1 and NFE-6. We demonstrate that NFE-6 contains C/EBPgamma, and address its role in globin gene regulation by erythroid overexpression of C/EBPgamma, and a dominant-negative form C/EBPgammaDeltaB, in mice. Elevated levels of C/EBPgamma, but not C/EBPgammaDeltaB, increase expression of the (fetal) gamma-globin relative to the (adult) beta-globin gene. Interestingly, fetal liver erythropoiesis is ablated when the C/EBPgamma and C/EBPgammaDeltaB levels are further increased in homozygous transgenics. We suggest that targeted expression of dominant-negative leucine zipper proteins is a generally applicable approach to ablate specific tissues in mice. |
