| First Author | Skowyra D | Year | 1999 |
| Journal | Science | Volume | 284 |
| Issue | 5414 | Pages | 662-5 |
| PubMed ID | 10213692 | Mgi Jnum | J:113807 |
| Mgi Id | MGI:3687688 | Doi | 10.1126/science.284.5414.662 |
| Citation | Skowyra D, et al. (1999) Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1. Science 284(5414):662-5 |
| abstractText | Control of cyclin levels is critical for proper cell cycle regulation. In yeast, the stability of the G1 cyclin Cln1 is controlled by phosphorylation-dependent ubiquitination. Here it is shown that this reaction can be reconstituted in vitro with an SCF E3 ubiquitin ligase complex. Phosphorylated Cln1 was ubiquitinated by SCF (Skp1-Cdc53-F-box protein) complexes containing the F-box protein Grr1, Rbx1, and the E2 Cdc34. Rbx1 promotes association of Cdc34 with Cdc53 and stimulates Cdc34 auto-ubiquitination in the context of Cdc53 or SCF complexes. Rbx1, which is also a component of the von Hippel-Lindau tumor suppressor complex, may define a previously unrecognized class of E3-associated proteins. |
