| First Author | Lim JM | Year | 2002 |
| Journal | J Cataract Refract Surg | Volume | 28 |
| Issue | 4 | Pages | 697-702 |
| PubMed ID | 11955914 | Mgi Jnum | J:143690 |
| Mgi Id | MGI:3828410 | Doi | 10.1016/s0886-3350(01)01236-6 |
| Citation | Lim JM, et al. (2002) Downregulated expression of ADAM9 in anterior polar cataracts. J Cataract Refract Surg 28(4):697-702 |
| abstractText | PURPOSE: To determine whether ADAM (a disintegrin and metalloproteinase) is regulated in lens epithelial cells (LECs) of patients with anterior polar cataracts and by transforming growth factor (TGF)-beta 1 in cultured LECs. SETTING: Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Seoul, Korea. METHODS: Lens epithelial cells attached to the anterior capsules of human cataractous lenses with nuclear and anterior subcapsular cataracts and noncataractous lenses were analyzed by reverse transcribed-polymerase chain reaction for the expression of ADAMs. The effect of TGF-beta 1 on ADAM gene expression was also tested in mouse lens epithelial explants and cultured LEC lines (alpha TN-4 and HLE B-3). RESULTS: Significantly reduced expression of mRNA for ADAM9 was observed in LECs from patients with anterior polar cataracts. The expression of mRNA for ADAM9 was downregulated by TGF-beta 1 in cultured human LECs. Treatment of cultured mouse LECs with TGF-beta 1 led to a reduction in ADAM1 mRNA. CONCLUSIONS: ADAMs are expressed and regulated in LECs. The downregulated expression of ADAM9 may serve as a marker for anterior polar cataracts in addition to previously known proteins, fibronectin, alpha-SMA, and beta ig-h3. The functions of this protein in lens pathology require further investigation. |
