| First Author | Seward ME | Year | 2008 |
| Journal | FEBS Lett | Volume | 582 |
| Issue | 17 | Pages | 2489-95 |
| PubMed ID | 18588881 | Mgi Jnum | J:201314 |
| Mgi Id | MGI:5512959 | Doi | 10.1016/j.febslet.2008.06.037 |
| Citation | Seward ME, et al. (2008) Flightless-I, a gelsolin family member and transcriptional regulator, preferentially binds directly to activated cytosolic CaMK-II. FEBS Lett 582(17):2489-95 |
| abstractText | In order to evaluate links between Ca2+/calmodulin (CaM)-dependent protein kinase type II (CaMK-II) and cell cycle progression, CaMK-II binding partners were sought in proliferating cells by epitope-tag tandem mass spectrometry. One protein identified was the gelsolin family member, flightless-I (Fli-I). Fli-I is not a CaMK-II substrate, but binds directly and preferentially to constitutively active (T287D) CaMK-II over inactive CaMK-II. Fli-I gradually enters the nucleus upon CaMK-II inhibition and is retained in the cytosol by T287D CaMK-II. CaMK-II inhibition and Fli-I overexpression suppress transcription of beta-catenin dependent transcriptional reporters, whereas Fli-I suppression enhances their transcription. These findings support a novel mechanism whereby cytosolic CaMK-II influences beta-catenin dependent gene expression through Fli-I. |
