| First Author | Kakumani PK | Year | 2020 |
| Journal | Life Sci Alliance | Volume | 3 |
| Issue | 4 | PubMed ID | 32161113 |
| Mgi Jnum | J:343145 | Mgi Id | MGI:7564181 |
| Doi | 10.26508/lsa.201900632 | Citation | Kakumani PK, et al. (2020) CSDE1 controls gene expression through the miRNA-mediated decay machinery. Life Sci Alliance 3(4) |
| abstractText | In animals, miRNAs are the most prevalent small non-coding RNA molecules controlling posttranscriptional gene regulation. The Argonaute proteins (AGO) mediate miRNA-guided gene silencing by recruiting multiple factors involved in translational repression, deadenylation, and decapping. Here, we report that CSDE1, an RNA-binding protein linked to stem cell maintenance and metastasis in cancer, interacts with AGO2 within miRNA-induced silencing complex and mediates gene silencing through its N-terminal domains. We show that CSDE1 interacts with LSM14A, a constituent of P-body assembly and further associates to the DCP1-DCP2 decapping complex, suggesting that CSDE1 could promote the decay of miRNA-induced silencing complex-targeted mRNAs. Together, our findings uncover a hitherto unknown mechanism used by CSDE1 in the control of gene expression mediated by the miRNA pathway. |
