| First Author | Sharma P | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 276 |
| Issue | 3 | Pages | 1320-4 |
| PubMed ID | 11027629 | Mgi Jnum | J:175119 |
| Mgi Id | MGI:5142385 | Doi | 10.1006/bbrc.2000.3606 |
| Citation | Sharma P, et al. (2000) Activation of LEDGF gene by thermal-and oxidative-stresses. Biochem Biophys Res Commun 276(3):1320-4 |
| abstractText | LEDGF promotes survival of many cell types against a wide range of environmental stresses, and cells responding to those stresses expressed higher levels of heat shock proteins (Hsps). LEDGF/p75 is a weak coactivator of general transcription. We speculated that a stress signal may activate expression of the LEDGF gene, resulting in elevated levels of LEDGF which may activate expression of stress-related proteins to protect cells from stresses. Lens epithelial cells (LECs) and cos7 cells were cultured under heat- or oxidative-stress. After cells were cultured for defined time, we quantified LEDGF mRNA and LEDGF protein with RT-PCR, Northern blot, and protein blot analysis. Our results showed that higher levels of LEDGF were found in both cell types under heat- and oxidative-stress than that in cells cultured at nonstress condition. Thus, one of the primary events in the stress-related protective events is the activation of LEDGF, a regulatory protein. |
